• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    A process for obtaining delipified human serum or plasma of low turbidity, and therefore decreased light scattering, is provided. This process comprises the simultaneous steps of deionization and pH adjustment of the serum or plasma to near the isoelectric point of lipoproteins commonly found in sera and results in the precipitation of much of the lipoprotein fraction. The process is carried out utilizing a mixed anion-cation exchange resin. The sera so obtained can be used as base material or a special diluent for verifying analytical information obtained in clinical analyses.
    公开号:SU928995A3
    申请号:SU802932151
    申请日:1980-06-03
    公开日:1982-05-15
    发明作者:Рей Бриггс Энглис；Милан Трис Джек
    申请人:Е.И.Дюпон Де Немур Энд Компани (Фирма)；
    IPC主号:
    专利说明:

    present in the whey to be processed. For complete exchange of anions and cations, it is necessary to use a sufficient amount of mixed ion exchange resin, as well as control the pH. The pH value corresponds to the isoelectric point of the lipoproteins normally present in the serum. In order to achieve a decrease in the pH value, which causes the precipitation of lipoproteins from the serum simultaneously with ion exchange, it is necessary to use an excess of cation exchange resin. It is necessary that an excess of cation exchange resin is introduced into the mixed anion cation exchange resin used. This excess amount of cation exchange resin is determined by titration of a given volume of serum, for example, 25 ml, diluted with (1, OOL) hydrochloric acid to pH. The amount of acid used for titration is referred to as the serum titer and, knowing this titer, use the appropriate amount of cation exchange resin. Frozen human serum is used to prepare the delipilized / serum. It is allowed to thaw at 32-37 ° C using a water bath. The ousted serum is examined to see if there are any visible signs of lipemia or hemolysis, i.e. the serum should be transparent and almost colorless. If the serum visually looks red or pink in color or is cloudy to the lumen, then this syrup is unsuitable for use. In addition, the serum should not contain pathogenic microorganisms. When the ion exchange resin and serum are mixed, a constant change in the pH value is carried out. In the first few minutes after adding the resin to the serum, the pH value increases from the original to 9.0 - 10.3. To decrease the pH and remove the ions from the serum. An excess of a mixed ion exchange resin is usually used in order to compensate for some decrease in the effectiveness of the resin caused by the deposition of lipoprotein sediment on the surface of the resin granules during the precipitation of lipoprotein. If necessary, 1,, 15 times more is added to the serum compared to the theoretically calculated amount of the mixed ion exchange resin. After the pH reaches 5.21: 0.3 (this usually takes 8-15 minutes |, mixing the ion exchange resin mixture and the sera are stopped and the supernatant serum (supernatant. tant) is separated from the resin and precipitated lipoproteins. The supernatant is separated from the precipitate by any known method, such as filtering, decanting, sucking off the supernatant serum. the salt is then adjusted to 4.0 t1.0 by adding hydrochloric acid to ensure low enzymatic activity in serum. It is desirable that the activity of the enzyme creatine kinase (CC does not exceed 3 IU / l. For this, the serum is kept at pH A, O to , 2 for a long time to ensure complete completion of denaturation. This process is controlled by periodically conducting a serum analysis to determine QC activity. This serum can be left overnight, stored at the final pH value, if stored at k C, the pH of the serum t to a final value of 7,, 3, by adding a suitable buffer solution, such as 2-amino 2-hydroxymethyl-1, 3 propane diol. This delimineed serum can be stored for a long time in the frozen state or for 48 hours (without the addition of microbial inhibitors). This serum can be used for clinical analysis. EXAMPLE Preparation of mixed anion-cation exchange resin. The duolit b-PC-301 universal high-capacity strong-acid cation-exchange resin and Duolit GPC-316 highly porous highly basic anion-exchange resin are mixed with each other, the mixture is thoroughly mixed to homogenize in a mixer until a homogeneous mixture is obtained that can be stored in tightly closed containers or containers that can be stored in sealed containers or containers that can be stored in sealed containers or containers that can be stored in sealed containers or containers that can be stored in sealed containers or containers that can be stored in sealed containers or containers that can be stored in sealed containers or containers that can be stored in sealed containers or containers that can be stored in sealed containers or containers that can be stored in sealed containers or containers that can be stored in sealed containers or containers that can be stored in containers and sealed containers or containers, which can be stored in sealed containers or containers, and they can be stored in containers and sealed containers or containers that can be stored in sealed containers. - dry weight and undesirable interaction with atmospheric carbon dioxide.
    权利要求:
    Claims (2)
    [1]
    Claim
    30 1. A method for producing delipilizirovannoy serum of animals by mixing the sera with anion exchange resins, characterized in that, with a view to higher degree delipilizatsii 35 Nia and separating electrolytes from the serum, the serum is mixed with anion-cation exchange resins, adjusting the pH to 4.9 “5.5, the supernatant is separated, the pH of the supernatant is adjusted to 3.0-5.0, the supernatant is maintained until the enzymatic activity decreases to 1-3 IU / L.
    [2]
    2. The method of pop. 1, differing ut 45 minutes and I with the fact that the supernatant pH adjusted to 6.7 "7.6 by addition of 2-amino-2-hydroxymethyl-1,3 'propanediol.
    50 Sources of information taken into account in the examination
    1. A. Noma. S imu 1 tatceoas Determination of Serum Cholesterol in Hiqh and Low Density Lipoproteins. - Cli5 $ nical Chem, 1978, v. .№ 24, p. 1504.
    • 3
    Circulation 717 Subscription
    Uzhhorod, st. Project, 4
    Branch of PPP Patent,
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    同族专利:
    公开号 | 公开日
    US4264471A|1981-04-28|
    EP0019939A1|1980-12-10|
    JPS6327660B2|1988-06-03|
    JPS55164360A|1980-12-22|
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    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    DE1617319C3|1951-01-28|1974-05-02|Biotest-Serum-Institut Gmbh, 6000 Frankfurt|
    DE1234417B|1963-05-04|1967-02-16|Merck Ag E|Test reagent for determining the fat content in organic substances, especially in blood serum|
    US3928566A|1970-08-14|1975-12-23|Du Pont|Lyophilized biological products|
    US3932943A|1970-08-14|1976-01-20|E. I. Du Pont De Nemours And Company|Method of preparation of lyophilized biological products|
    US3682835A|1970-11-24|1972-08-08|Baxter Laboratories Inc|Liquid blood serum control standard|
    US3706660A|1971-03-25|1972-12-19|Squibb & Sons Inc|Process for removal of particle-forming proteins from blood sera|
    US4007008A|1975-07-30|1977-02-08|Becker Milton J|Preparation of reference serum from animal blood|
    US4039285A|1975-10-10|1977-08-02|Ortho Diagnostics, Inc.|Single sample method for determination of lipoprotein concentrations in blood|
    NZ180199A|1976-03-04|1978-11-13|New Zealand Dev Finance|Method of testing for the presence of elevated plasma liprotein concentration|
    DE2723070C2|1976-05-24|1986-03-06|Walter Philadelphia Pa. Brummund jun.|Reference preparation for determining a halide or carbonate concentration in chemical analyzes|
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    US4185963A|1977-10-03|1980-01-29|Conoco Inc|Method for determining lipids in blood serum|
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    DE3107060A1|1981-02-25|1982-09-09|Boehringer Mannheim Gmbh, 6800 Mannheim|CONTROL OR CALIBRATION SERUM AND METHOD FOR THE PRODUCTION THEREOF|
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    US06/045,182|US4264471A|1979-06-04|1979-06-04|Serum and plasma clarification process|
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